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KMID : 0861020220370050053
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Korea Journal of Herbology
2022 Volume.37 No. 5 p.53 ~ p.61
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Effects of Baicalein on hydrogen peroxide productions in RAW 264.7 macrophages stimulated by lipoteichoic acid
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Oh Chi-Seok
Park Wan-Su
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Abstract
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Objectives : The aim of this study was to investigate the effect of Baicalein (BA) on the production of hydrogen
peroxide in lipoteichoic acid-stimulated RAW 264.7 mouse macrophages.
Methods : Lipoteichoic acid-stressed RAW 264.7 mouse macrophages were incubated with baicalein at concentrations
of 50 and 100 μM. Incubation time is 30 minutes, 2 h, 12 h, and 18 h. After incubation, The production of hydrogen
peroxide in RAW 264.7 mouse macrophages was measured with dihydrorhodamine 123 assay. Streptococcus aureus
lipoteichoic acid and Streptococcus pyogenes lipoteichoic acid were used as cell-stimulating lipoteichoic acid. Cell
viabilities were measured with a modified MTT assay. Berberine, indomethacin, and gallic acid were incubated for
the same time as the comparative materials.
Results : BA at the concentration of 50 and 100 μM did not show cytotoxicity on RAW 264.7 mouse macrophages for
24 h incubation. For 30 minutes, 2 h, 12 h, and 18 h incubation, BA at the concentration of 50 and 100 μM significantly
inhibited the production of hydrogen peroxide in RAW 264.7 mouse macrophages stimulated by Streptococcus aureus lipoteichoic acid (p < 0.05); also, BA at the concentration of 50 and 100 μM also inhibited the productions of
hydrogen peroxide in RAW 264.7 mouse macrophages stimulated by Streptococcus pyogenes lipoteichoic acid significantly
(p < 0.05).
Conclusions : BA might have anti-bacterial activity related to its inhibition of hydrogen peroxide production in lipoteichoic
acid-stimulated RAW 264.7 mouse macrophages
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KEYWORD
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RAW 264.7, Baicalein, Macrophage, Lipoteichoic acid, Hydrogen peroxide
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